Poly(A)-ClickSeq for Differential Gene Expression

We developed Poly(A)-ClickSeq (PAC-Seq) as a sequencing platform that allows for simultaneous mapping of poly(A) sites within mRNA and for the measurement of differential expression of genes. To illustrate the utility of PAC-Seq, we depleted a component of the Drosophila Integrator complex in S2 cells using RNAi and compared the gene expression changes relative to control dsRNA-treated cells. RNA isolated from these cells was then subject to standard RNA-seq or PAC-seq.