Transcriptome effects mediated by forced overexpression of BACE1-AS in HAOEC cells

Recently, high-throughput studies identified tens of thousands of sites transcribed to produce transcripts with little protein-coding potential. The function of these transcripts remains largely obscure and their relevance to cardiovascular disease is mostly undefined. Many non-protein-coding transcripts belong to the group of Long non coding RNAs (LncRNAs), which are arbitrarily classified according to their length >200 nt. They control protein targeting to genomic loci, epigenetic silencing and serve as scaffolds for multiple proteins.We measured the expression of 83 diseases-related LncRNAs in biopsies from heart failured patients and in controls. We found 15 lncRNAs modulated in HF samples. To explore the functional relevance of two of them, H19 and BACE1-AS, we investigated the effects of their forced overexpression on transcriptome. BACE1-AS were overexpressed by pREceiver-Lv-105 (GeneCopoeia) lentivirus expressing BACE1-AS human cDNA in human aorta endothelial cells (HAOEC). We measured, by llumina HumanHT-12 v4 BeadChip platform, the transcriptome changes induced by the forced overexpression of the LncRNA compared to a negative control (empty vector).