Schematic Representation of the A. CHIKV and B. DENV genomes, respectively, with regions targeted by the Dual Targeting Introns.

A. CHIKV genomic RNA diagram with the indicated conserved region targeted for viral suppression. The CHIKV conserved domain, labeled as the CHIKV Conserved Region, was determined through a Clustal X alignment of GenBank obtained CHIKV sequences (see Materials and Methods and S5 Fig). B. The most invariant segments of the DENV RNA are known as the 5’ and 3’ cyclization sequences (labeled as the Group I Intron target site). These sequences are so named because they are complementary to each other and are thought to be involved in the formation of a panhandle structure during genome replication.