Pseudomonas aeruginosa PAO1 Genome sequencing and assembly. Pseudomonas aeruginosa PAO1

5mL PAO1 culture was mixed with PaoP5(dap1 mutant) or PaoP5 (MOI=10) for 10 min, and 5mL PAO1(lon mutant) was infected with aoP5(dap1 mutant) (MOI=10) for 10 min. The 1ml sample was then made into pellets. proteomic analysis was performed by Applied Protein Technologies. Biological replication tests were performed 3 times in each group. The samples were lysed with SDT buffer and quantified with BCA protein assay kit. The protein is digested with trypsin and then desalted with a C18 cartridge. The digested peptides were concentrated by vacuum centrifuge, recombined with formic acid, and then analyzed by LC-MS/MS. The peptides were uploaded to a reversed-phase column and connected to a C18 reversed-phase analysis column of formic acid, separated by a linear gradient buffer containing acetonitrile and formic acid at a flow rate of 300 nL/min. The mass spectrometer operates in positive ion mode. The original MS data of each sample were combined, and MaxQuant 1.5.3.17 software was used for identification and quantitative analysis. Protein sequences of differentially expressed proteins (DEPs) were retrieved using NCBI BLAST.