Expression of cyclophilin B is associated with malignant progression and regulation of genes

Microarray was conducted on Illumina Human Ref-6 Version 2 Expression Chip (Illumina, San Diego, CA). Three independent cultures were used for RNA isolation with RNeasy plus mini kit (Qiagen, Valencia, CA). RNA quality was checked by Agilent Bioanalyzer (Agilent, Santa Clara, CA). An Ambion labeling kit was used for labeling cDNA followed by hybridization to Illumina chips. ChIP scan data was extracted by Illumina Beadstudio and subsequently analyzed using Bioconductor lumi package. T47D cells (si-Luc and si-CypB cells) were cultured in the growth medium for three days followed by 24 hours arrest prior to PRL treatment (100 ng/ml) for 2 hours.