Single cell RNA-seq of intestinal L cells in antibiotic-treated and Desulfovibrio-colonized mice

To examine the effects of Desulfovibrio on L cells, L cells from antibiotic-treated and Desulfovibrio-colonized B6.Cg-Gcgtm1.1(cre/GFP)Rpa/J (Gcg-Cre:GFP) mice were isolated and subjected to scRNA-seq. Overall design: Gcg-Cre:GFP mice were treated with an antibiotic cocktail, followed by Desulfovibrio colonization. After 2 weeks, L cells (GFP+) were isolated by FACS sorting, stained with Hashtag antibodies and subjected to single cell (sc)RNA sequencing with the 10x Genomics platform. L cells in this analysis represent cells from two different treatment conditions: Cells from mice treated with an antibiotic cocktail Cells from mice colonized with Desulfovibrio