Proteomic analysis of paclitaxel-tolerant ovarian cancer OVCAR8-Persister cells revealed a metabolic shift to fatty acid oxidation

Human ovarian adenocarcinoma OVCAR8 cells and paclitaxel-induced OVCAR8 -Persister cells (3 biological replicates) were collected in lysis buffer and then sonicated three times on ice. The supernatant represented the whole-cell extract. 4D-data independent acquisition (DIA)-based label-free quantitative proteomics was performed on a timsTOF Pro2 (Bruker Daltonics, USA) mass spectrometer coupled to a nanoElute UHPLC system (Bruker Daltonics, USA).