Transcriptome analysis in calorie-restricted rats implicates epigenetic and post-translational mechanisms in neuroprotection and aging

Using RNA-seq, 39 cerebral cortex RNA samples were sequenced. The study design was as follows: Ad libitum fed rats at 6 months (n=3, 6 individuals pooled), 12 months (n=3, 6 individuals pooled) and 28 months (n=3, 6 individuals pooled). Calorie restricted rats at 6 months (n=3, 6 individuals pooled), 12 months (n=3, 6 individuals pooled) and 28 months (n=3, 6 individuals pooled). Rats fed alpha lipoic acid as a supplement to ad libitum at 12 months (n=3, 6 individuals pooled) and 28 months (n=3, 6 individuals pooled). Diet switching groups, where diet was changes at 12 months; 28 month ad libitum switched to calorie restriction (n=3, 6 individuals pooled), 28 month calorie restriction switched to ad libitum (n=3, 6 individuals pooled), 28 month ad libitum plus lipoic acid switched to calorie restriction (n=3, 6 individuals pooled), 28 month calorie restriction switched to ad libitum plus lipoic acid (n=3, 6 individuals pooled).本研究通过RNA测序（RNA-seq）对39份大鼠大脑皮层RNA样本进行了测序。研究设计如下：自由进食组大鼠，包括6月龄（n=3，每3份样本由6只大鼠个体混合制备）、12月龄（n=3，每3份样本由6只大鼠个体混合制备）及28月龄（n=3，每3份样本由6只大鼠个体混合制备）的个体；热量限制组大鼠，包括6月龄（n=3，每3份样本由6只大鼠个体混合制备）、12月龄（n=3，每3份样本由6只大鼠个体混合制备）及28月龄（n=3，每3份样本由6只大鼠个体混合制备）的个体；自由进食补充α-硫辛酸组大鼠，包括12月龄（n=3，每3份样本由6只大鼠个体混合制备）及28月龄（n=3，每3份样本由6只大鼠个体混合制备）的个体；饮食转换组大鼠于12月龄时调整饮食方案，具体包括：28月龄时由自由进食转换为热量限制（n=3，每3份样本由6只大鼠个体混合制备）、28月龄时由热量限制转换为自由进食（n=3，每3份样本由6只大鼠个体混合制备）、28月龄时由自由进食联合α-硫辛酸转换为热量限制（n=3，每3份样本由6只大鼠个体混合制备）、28月龄时由热量限制转换为自由进食联合α-硫辛酸（n=3，每3份样本由6只大鼠个体混合制备）。