Genome-wide DNA-binding analysis for Sox100B in intestinal stem cells by DamID

Purpose: Genome-wide DNA-binding analysis for Sox100B in intestinal stem cells in Drosophila midgut by DNA adenine methyltransferase identification(DamID) Methods: DNA adenine methyltransferase identification (DamID) on Sox100B driven by ESG-Gal4 Results: 5046 significant genomic binding sites, corresponding to 3182 genes were identified via iDamID following Sox100B overexpression in progenitor cells compared to WT. About a third of these genes were in the list of significantly altered genes following Sox100B overexpression and/or depletion. Overall design: Dam(Ctrl) or Dam-Sox100B transgenes were expressed in intestinal stem cells by ESG-Gal4.The guts with 2 days after transgene expression were used for DamID-seq, followed by deep sequencing analysis.