Comparison of gene expression quantification using a custom qPCR mix and Maxima® SYBR Green Master Mix.
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cDNA (1 100 dilution) synthesized using a 20 µL reaction iScript™ cDNA synthesis and quantified in a 10 µL qPCR reaction using Maxima® SYBR Green Master Mix or custom made master mix. Values represent the fold difference between biofilm and planktonic population plus or minus standard deviation of 3 independent experiments. Values above 1 indicate up-regulation in biofilm, and below 1 indicates down-regulation.*Statistical differences (pN/D – non detected.
创建时间：
2015-12-02
