Dynamic changes in chromatin states during specification and differentiation of adult intestinal stem cells [rnaseq]
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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To follow the changes in the transcriptional programs accompanying the specification, maintenance and differentiation of the adult ISCs we sequenced whole transcriptomes of embryonic intestinal epithelium progenitors (at E12.5 and E14.5), adult ISCs and their differentiated progenies, the majority of which are absorptive enterocytes. EpCAM positive embryonic gut epithelium was isolated from dissected small intestines using fluorescence activated cell sorting (FACS). Adult ISCs were purified on the basis of GFP fluorescence from crypts of Lgr5GFP-Cre-ERT mice (Barker et al. 2007), whereas enterocytes (EpCAM+ CD45- CD31-) were isolated from villi.
提供机构：
Institute of Molecular Biology, Mainz
创建时间：
2022-02-20
