Evaluation of the role of Fbxw7 in interferon-gamma (Ifng) signaling in tumor cells

RNA-sequencing followed by gene set enrichment analysis was used to identify pathways enriched or depleted in Fbxw7-deficient cells. We found that virus sensing, as well as type I and II IFN signaling pathways, were among the most significantly downregulated gene sets in Fbxw7-deficient cells compared with controls. These results establish Fbxw7 as a regulator of the dsRNA sensing and IFN signaling pathways. Overall design: Fbxw7-deficient and control melanoma cells were treated with Ifng (10ng/mL) for 24h. Total RNA was extracted and submitted to the Molecular Biology Core Facility at Dana-Farber Cancer Institute for sequencing.