Validation of microarray data by qPCR and protein levels.

Mouse bone marrow cells were differentiated in GM-CSF for 6 days in the presence of 200 nM sTM from day 4 to 6. DCs were then analyzed for the expression of cell surface markers by flow cytometry while RNA was analyzed on microarrays and by qPCR. In some experiments protein levels in the medium was determined by ELISA. The direction of change in levels of protein is represented by arrows. CD206 is also known as mannose receptor C type 1 (MRC1). The mRNA data shows the results for both the A and B chains of IL-12p70. ND: not determined; the value of the change in gene expression in qPCR was determined by ratio of ΔΔct. The FACS and ELISA data were taken from Takagi et al [11].