Disrupting KIF20A activity inhibits the meiosis competence and cell cycle progression of porcine oocytes.

(A) Granulosa cell diffusion of COCs was inhibited during meiosis. Almost all of COCs in control groups were surrounded by more than 5 layers of intact cumulus cells, whereas the COCs in 10, 20 and 50 µM Paprotrain treatment groups showed weakened diffusion. (B) Rates of first polar body extrusion of porcine oocytes after 44 h culture with different treatments (Control, 10 µM, 20 µM, and 50 µM). Oocytes failed to extrude the first polar body after treatment with KIF20A inhibitor. * significant difference (p<0.05). (C) Effects of KIF20A disruption on cell cycle during oocyte meiosis maturation. The cell cycle progression of porcine oocytes was disturbed during meiosis. The oocytes arrested at GVBD or MI stage and cannot perform the proper progression of cell cycle after KIF20A disruption. * significant difference (p<0.05).