Inflammatory cytokine and prostacyclin release from HMVEC after stimulation of NOD1 or TLR4.

HMVEC were cultured for 24 hours in 96 well plates with media alone (CTRL), iE-DAP 10 µg/ml (NOD1) or LPS 1 µg/ml (TLR4). (A) Inflammatory cytokine release measured using the Human Pro-Inflammatory 9-PlexBase Kit from Meso Scale Discovery (MSD®). Results are expressed as mean ± SEM for n = 15. (B) 6-keto prostaglandin F1α release (breakdown product of Prostacyclin) measured by ELISA. Results are expressed as mean ± SEM for n = 15. Results analysed by one-way ANOVA with Dunnett’s multiple comparison test (*P<0.05) or by Kruskal-Wallis test with Dunn’s multiple comparison test for non-parametric data (#P<0.05).