The neocortex contains an unparalleled diversity of neuronal subtypes responsible for complex behavior. Each cortical neuron has distinct traits, which are developmentally acquired under the control o
siRNA transfections. In 96-well plates, 25 µL of siRNA at 30 nM in Opti-MEM (Thermo Fisher 31985-062) was added per well, followed by 25 µL of transfection reagent (Thermo Fisher Lipofectamine RNAiMAX