Ectopic expression of Ino80 complex subunit Actr5 in mouse heart

Actr5 is one of the subunits of the ATPase-dependent chromatin remodeling complex INO80. In muscle tissues such as skeletal muscle, heart, and aorta, the expression of Actr5 is much lower than that in non-muscle tissues. During skeletal and smooth muscle development, it interacts with transcription factors such as MyoD, MyoG, SRF, and myocardin to play a repressive role in muscle differentiation, but its physiological role in cardiac development is not entirely clear. To investigate the role of Actr5 in the heart, AAV6 expression vectors containing Actr5 gene were infected into mice, and total RNA were extracted from the heart, followed by DNA microarray analysis. Three-week-old mice were injected intraperitoneally with the Actr5-AAV6 vector. As a control, empty AAV6 vector was injected. Three weeks after injection, hearts were harvested and RNA was extracted from them.