Tumor-associated and control mammary adipose tissue in lean and high fat diet-induced obese mice

To investigate the crosstalk between a growing breast cancer tumor and adjacent adipose tissue, we set up an breast cancer mouse model and compared the gene expression between control and tumor-associated adipose tissue. Overall design: Female mice, fed either regular chow or high fat diet for 16 weeks, were injected with E0771 cancer cells resuspended in PBS mixed with Matrigel into their left inguinal/mammary fat pad (tumor-associated inguinal adipose tissue) while their right inguinal fat pad (control inguinal adipose tissue) received PBS-Matrigel solution alone. Tumor-associated and control IWAT were collected two weeks after cancer cell injections.