Metagenome from Paralichthys olivaceus. food metagenome

Food-poisoning fillet of Paralichthys olivaceus. Total DNA or RNA was prepared from a 30 mg of the frozen fillet of Paralichthys olivaceus by RecoverAll Total Nucleic Acid isolation kit (Ambion, Austin, TX USA). Double-stranded cDNA was prepared from 1 micro g of total RNA using the random priming method with SuperScript Choice System for cDNA synthesis (Invitrogen, Carlsbad, CA, USA). cDNA was purified using a QIAquick PCR Purification kit (QIAGEN, Hilden, Germany). Short-read DNA sequencing using the Illumina Genome Analyzer II An approximately 100-bp length DNA library was prepared from the mixture of 2 micro g of total DNA and 0.5 micro g of cDNA using a genomic DNA sample prep kit (Illumina, San Diego, CA, USA), and DNA clusters were generated on a slide using a Single Read Cluster Generation kit (ver. 2) on an Illumina cluster station (Illumina), according to the manufacturer's instructions.