Expression data from Luteolin-treated human Neural Stem Cells (hNSCs)

Gene expression profiling reveals a potential role of Luteolin in human neuronal stem cells (hNSCs) differentiation . hNSCs purchased from Gibco were treated with 1 μM verbenalin for 24 hours. Microarray gene expression profiling was conducted for biological replicates of hNSCs cultured in differentiation cell culture medium supplemented with Luteolin for 24 hours and untreated control cells cultured in differentiation cell cultured medium . RNA was extracted using Isogen (Nippon Gene Co. Ltd., Toyama, Japan). The integrity of RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). RNA samples were prepared for gene expression profiling analysis with GeneChip® 3' Expression Arrays using 3' IVT PLUS Reagent Kit (Affymetrix Inc., Santa Clara, CA, USA). One hundred ng of total RNA from each sample was used to generate amplified and biotinylated complementary RNA (cRNA) from poly (A) RNA in a total RNA sample according to the user manual. IVT Incubation time was 16 hour. GeneAtlas® Hybridization, Wash and Stain Kit was used for hybridizing 3' IVT Array Strips according to the user manual (P/N 08-0306). Human genome array strips (HG-U219) were hybridized for 16 hours in a 45oC incubator, washed and stained and finally imaging was done with the GeneAtlas Fluidics and Imaging Station.