Detection of Polyvinylpyrrolidone in Daphnia magna: Development of a refractive index quantification method for water-soluble polymers in aquatic organisms: data

The water-soluble polymer polyvinylpyrrolidone (PVP) is an established ingredient in pharmaceutical and personal care product (PPCP) formulations. Due to its high usage and lack of biodegradability, it has been detected up to 7.0 mg L-1 in wastewater and 0.1 mg L-1 in the receiving freshwaters, with several studies showing detrimental sublethal effects in a range of aquatic species. A lack of simple analytical methods to detect and quantify PVP currently impacts further investigation into the cause of these sublethal effects. In this paper we propose a refractive index gel-permeation chromatography (GPC) method to quantify PVP, which includes the processing of raw chromatograms using line deconvolution to calculate peak area. The method was then applied to Daphnia magna exposed to PVP for 48 hours. A limit of detection (LOD) and limit of quantification (LOQ) of 0.05 and 0.2 mg mL-1 respectively was determined, with a recovery of 78% from spiked Daphnia magna. PVP was detected in the samples above the LOD but below the LOQ. This suggests PVP is ingested by Daphnia magna, which warrants further investigation into whether bioaccumulation of PVP could be causing the sublethal effects seen in other studies.The dataset includes GPC data to describe molecular weights of polymers and lineshape deconvolution data in Excel format. Original data files from Fityk are also included.Research results based upon these data are publisehd at https://doi.org/10.1016/j.envpol.2024.123431

水溶性聚合物聚乙烯吡咯烷酮（PVP）是药品和个人护理产品（PPCP）配方中的既定成分。鉴于其广泛的应用及其生物降解性的缺乏，PVP在水体废液中已检测到高达7.0 mg L-1，在接收的淡水中为0.1 mg L-1，多项研究表明，在多种水生生物中产生了有害的非致死效应。目前缺乏简单的分析方法来检测和量化PVP，这影响了对这些非致死效应原因的进一步研究。在本研究中，我们提出了一种折射率凝胶渗透色谱法（GPC）来量化PVP，该方法包括使用线去卷积处理原始色谱图以计算峰面积。然后将此方法应用于暴露于PVP中48小时的斑马鱼。确定了检测限（LOD）和定量限（LOQ）分别为0.05和0.2 mg mL-1，从添加斑马鱼的样品中回收率为78%。在检测到的样品中，PVP的浓度高于LOD但低于LOQ。这表明斑马鱼摄入了PVP，这为探究PVP的生物积累是否导致了其他研究中观察到的非致死效应提供了进一步研究的理由。数据集包括用于描述聚合物分子量的GPC数据和线条去卷积数据，格式为Excel。还包括来自Fityk的原始数据文件。基于这些数据的研究成果已发表在https://doi.org/10.1016/j.envpol.2024.123431。