Next Generation Sequencing Facilitates Quantitative Analysis of Ern1f/f and Ern1f/f AKO iWAT transcriptomes

Bulk RNA-seq has uncovered the the function of adipocyte IRE1a in cold-induced thermogenic program in inguinal white adipose tissue. The GO analysis shows that adipocyte IRE1a supressed inflammatory responds when the mice were maintained at 30oC and adipocyte IRE1a abrogation facilated cold-induced thermogenic program after challenged with cold stress. In addition, the transcription factor (TF) analysis indicates that adipocyte IRE1a repressed lots of temperature-dependent TFs, including PGC1a. Overall design: There are 4 groups (AKO30C, AKO8C, WT30C, WT8C) and 3 mice each group.