Long non-coding RNA dysregulation is a frequent event in non-small cell lung carcinoma pathogenesis. Long non-coding RNA dysregulation is a frequent event in non-small cell lung carcinoma pathogenesis

Our aim was to contribute to a more comprehensive landscape for lncRNA deregulation in NSCLC by identifying lncRNA profiles that could significantly discriminate NSCLCs tissue from paired normal lung tissue. Therefore, we assessed the transcriptional profile of 44 paired tumour and adjacent normal lung tissue from NSCLC patients using an Agilent Custom 8x60K expression array (AMADID: 047718) designed by the GENCODE consortium. This microarray, based on the Gencode v15 catalog of human lncRNA, interrogates 17,535 randomly selected protein coding and 22,001 lncRNA transcripts, being each transcript targeted by two probes. Overall design: Two-condition experiment, NSCLC vs. adjacent normal tissue. Biological replicates: 44 tumour and adjacent normal tissue pairs. Technical replicates: 3 tumour and adjacent normal tissue pairs.