Transcriptomic analysis of cardiomyocyte upon OSKM induction

We generated i4F (Heart) mice which has cardiac-specific and doxycycline (DOX) controllable OSKM expression system. Using next generation sequencing (NGS), we analyzed the whole transcriptomic profiles in isolated adult cardiomyocytes from i4F (Heart) mice, which were under different DOX-treatments. We found the i4F (Heart) mice upon 6 days and 12 days DOX their cardiomycytes had remarkable change of transcriptome to be like neonatal and embryonic cardiomyocytes, which exhibited dedifferentiation, significant change in metabolism, and cell-cycle reactivation. While cardiomyocytes showed molecular re-differentiation when DOX withdrawn after 6 days treatment. Overall design: Total RNA of isolated cardiomocytes were extracted from 12-16 weeks old i4F (Heart) mice. Those mice had different DOX treatments, DOX- (no DOX), DOX6 (6 days of DOX), DOX12 (12 days of DOX), DOX6Water6 (6 days DOX and no DOX for additional 6 days). Two biological replicates were profiled for each cell type.