Divergence in testis transcriptome between Japanese sticklebacks (CBX247). unidentified

We compared transcriptome between nesting males of Japan Sea sticklebacks and Pacific Ocean sticklebacks. Testes were collected from four nesting Japan Sea males and four nesting Pacific Ocean males. Total RNA was isolated with Trizol Reagent (Invitrogen, Carlsbad, CA, USA), followed by purification with RNeasy Micro Kit (Qiagen, Valencia, CA, USA). RNA from each fish (n = 8) was labeled with Cy3 and hybridized separately to a custom-made microarray (n = 8 arrays) created by Agilent Technologies (Santa Clara, CA, USA). RNA from different fishes were not pooled, so there are four biological replications of each form. Agilent custum array (8X60K) was used.