ChIP-seq analysis for HDAC2 in Caco2 cells infected with vector or PIM1

PIM1 is constitutively active and involved in various cellular processes, including cell survival, proliferation, and differentiation by phosphorylating a wide range of substrates. We investigated whether PIM1 is involved in the pathogenesis of ulcerative colitis (UC).We performed ChIP-seq analysis for HDAC2 to study the molecular mechanisms through which PIM1 regulates goblet cell numbers. ChIP-seq analysis for HDAC2 in Caco2 cells infected with vector or PIM1.The ChIP and input DNA fragments were processed for end repair and A-tailing using the NEBNext End Repair/dA-Tailing Module (E7442, NEB)