Murine Prostate Tissue: Rb1 null vs. Rb1 E2F binding deficient mutant

Transcriptional profilling of 60 day old prostate tissue from Rb1F/F:Trp53F/F:PB-Cre4 or Rb1R654W/F:Trp53F/F:PB-cre4 mice versus wild type prostate tissue. Profiles used to compare deregulation of known E2F target genes in prostate tissue expressing a mutant Rb1 gene to tissue lacking Rb1 expression. Tissue from two Rb1F/F:Trp53F/F:PB-Cre4 (null) mice or two Rb1654/F:Trp53F/F:PB-cre4 (mutant) mice compared to a pool of tissue from 3 wild type mice using two color arrays.本数据集针对60日龄Rb1F/F:Trp53F/F:PB-Cre4或Rb1R654W/F:Trp53F/F:PB-cre4小鼠的前列腺组织，与野生型前列腺组织开展转录谱分析（Transcriptional profiling）。该转录谱用于对比表达突变型Rb1基因的前列腺组织与缺失Rb1表达的组织中，已知E2F靶基因（E2F target genes）的表达失调情况。本研究选取2只Rb1F/F:Trp53F/F:PB-Cre4（敲除型，null）小鼠、2只Rb1654/F:Trp53F/F:PB-cre4（突变型）小鼠的组织，与3只野生型小鼠的混合组织，通过双色基因芯片（two color arrays）进行对照分析。