Structure of methanogenic communities and methane emissions from enriched Amazonia soil under distinct land use

Here, we aimed to investigate the composition and structure of bacterial and archaeal communities in tropical soils in response to land-use changes, emphasizing the methanogenic communities. We collected soil samples from primary forest, pasture, and secondary forest of the Amazonia region. We used a strategy based on the enrichment of the methanogenic community, followed by the measurement of methane emission, quantification of mcrA gene copies by qPCR, and total 16S rRNA gene sequencing (metataxonomics).