Fluid shear stress-dependent modulation of the basal endothelial glycocalyx

The vascular endothelial glycocalyx is a major regulator of endothelium function, serving as a vital mechanotransducer and barrier. Shear stress due to blood flow exerted on the apical endothelial cell (EC) and glycocalyx has been the primary focus of the field due to their direct interaction. Recently, it has been demonstrated that the basal glycocalyx, exposed to the basement membrane, is sensitive to apical fluid shear stress and necessary for promoting mechanotransduction pathways key to the EC response to blood flow. Here, we demonstrate the fluid shear stress-dependent modulation of the basal endothelial glycocalyx, showing a notably different expression pattern and overall increased expression compared to the apical glycocalyx. The dataset found here includes the raw confocal z-stacks collected of fluorescently tagged human microvascular endothelial cells after fluid shear stress exposure and the associated raw data from quantitative analysis of the immunofluorescent signal. Our ..., , # Fluid shear stress-dependent modulation of the basal endothelial glycocalyx

Dataset DOI: [10.5061/dryad.c59zw3rn1](https://doi.org/10.5061/dryad.c59zw3rn1)

## Description of the data and file structure

The following folders contain raw images, code, and data files relating to our study: \"Fluid shear stress-dependent modulation of the basal endothelial glycocalyx,\" published in PLOS ONE.

The raw z-stacks collected and analyzed are within the \"Images\" folder. The images are separated first by the shear rate the HLMVECs experienced (static (0), 0.5, 10, or 30 dynes/cm^2^), and then secondly by the exposure time (0.5 or 12 hours), and permeabilization status for the static and 10 dynes/cm^2^ groups. 

Within the \"Code\" folder is the ImageJ macro used to separate the z-stacks collected into apical and basal sum projections (\"BasalWGA_AB_StackProjGen\") and the Cell Profiler pipelines used to segment and quantify glycocalyx signal in terms of integrated intensity, coverage, and thickness...,