16S analysis of gut microbiota diversity Metagenome

After 7 days of adaptive feeding, 18 male C57BL/6J mice aged 3 months were weighed and randomly divided into three groups: Control group, Model group (72 hours of sleep deprivation), and Sch B group (Schisandrin B+72 hours of sleep deprivation). Seven days before sleep deprivation, Sch B group was orally administered (Sch B, 40mg/kg), while Control group and Model group were orally administered with equal volumes of drinking water. After 7 days of gavage, all groups except for the Control group were subjected to 3 days of sleep deprivation. After sleep deprivation, the colon contents of each mouse were collected for 16S microbial diversity analysis.