SLC26A4-AS1 Aggravates AngII-induced Cardiac Hypertrophy by Enhancing SLC26A4 Expression

Abstract Background It has been reported that solute carrier family 26 members 4 antisense RNA 1 (SLC26A4-AS1) is highly related to cardiac hypertrophy. Objective This research aims to investigate the role and specific mechanism of SLC26A4-AS1 in cardiac hypertrophy, providing a novel marker for cardiac hypertrophy treatment. Methods Angiotensin II (AngII) was infused into neonatal mouse ventricular cardiomyocytes (NMVCs) to induce cardiac hypertrophy. Gene expression was detected by quantitative real-time PCR (RT-qPCR). Protein levels were evaluated via western blot. Functional assays analyzed the role of SLC26A4-AS1. The mechanism of SLC26A4-AS1 was assessed by RNA-binding protein immunoprecipitation (RIP), RNA pull-down, and luciferase reporter assays. The P value

摘要 背景 已有研究表明，溶质转运蛋白家族26成员4反义RNA1（SLC26A4-AS1）与心脏肥厚密切相关。研究目的 本研究的目的是探究SLC26A4-AS1在心脏肥厚中的作用及其特定机制，为心脏肥厚治疗提供一种新的标志物。研究方法 通过向新生小鼠心室心肌细胞（NMVCs）注射血管紧张素II（AngII）以诱导心脏肥厚。采用定量实时PCR（RT-qPCR）检测基因表达。通过蛋白质印迹法评估蛋白质水平。功能实验分析了SLC26A4-AS1的作用。通过RNA结合蛋白免疫沉淀（RIP）、RNA捕获和荧光素酶报告基因实验评估了SLC26A4-AS1的机制。P值