Single Nucleotide Resolution Analysis of Nucleotide Excision Repair of Ribosomal DNA in Humans and Mice

In the present work we have applied analytical methods to map repair events in rDNA using data generated by the newly developed XR-seq genome-wide single nucleotide repair technology. We find that in human and mouse cell lines, rDNA is not subject to TCR of damage caused by UV or by cisplatin. Overall design: We perform XR-seq in mouse skin fibroblast under UV irradiation and collect cells after incubation 3 hours. For human cell lines NHF1, CSB and XPC, we perform XR-seq under UV irradiation and collect cells after incubation 1 hour. For GM12878, we perform XR-seq under cisplatin and collect cells after incubation 2 hours. Then we mapped all the reads to rDNA or DHFR. This dataset includes re-analysis of five GSE67941 Samples and two GSE82213 Samples.