Maternal KLF17 regulates zygotic genome activation in the mouse

To investigate the change of embryo transcriptome upon the inhibition of transition, we treated the late zygote with CHX and performed RNA-seq; To test KLF17 necessity for ZGA transcripts expression and embryo development, we next generated conditional knockout mouse models for Klf17 and collected embryo to perform RNA-seq and Stacc-seq. We selected seven stages of oocyte and embryo when we treated embyo with CHX; For the transcriptome of maternal Klf17 knockout embryo, We selected two stages of embryo; For the Stacc-seq of maternal Klf17 knockout embryo, only early 2 cell stage of embryo was used.