RNA-Seq of zebrafish whole-eye comparing wild-type and mab21l1 c.107delA p.(Lys36Argfs*7) embryos at 5 days post fertilization

Currently there is little knowledge on the functions and signaling pathways of MAB21L1. Therefore, we developed a zebrafish genetic model of mab21l1 deficiency to use in order to gain insight into possibly affected pathways and transcripts. We conducted RNA-seq on zebrafish whole eyes which were dissected out at 5 days post fertilization. Three biological replicates per genotype (wild-type or homozygous mab21l1-c.107delA) were collected, with 50 eyes per replicate. RNA was extracted and purified and submitted to Macrogen to conduct RNA-Sequencing procedure.