Lightsheet dataset of midgut morphogenesis in w, 48Y-GAL4; klar x w; UAS-histRFP embryo TIFF of pullback images, each image taken 60 seconds apart spcutMeshSmRS_0000XX.mat files provide the
In 2015, we launched the mesoSPIM initiative (www.mesospim.org), an open-source project for making light-sheet microscopy of large cleared tissues more accessible. Meanwhile, the demand for imaging la
This data is a tiff datatype file simulating lightsheet images acquired by MAXWELL, with known variables. The simulated data employs a 230 nm in-plane imaging pixel size, which imitates a 20x magnific
We present an optimized tissue clearing procedure that preserves fluorescence markers and enables immunolabeling in tissue such as mouse sciatic nerve, gut and spinal cord. By refining the original pr
