E. coli K12 and Bacteriophage λ Strains.
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aThe λ prophage genes int-xis-exo-bet-gam-kil in strain Y836 were substituted with bio275 [13]. The strain carries the chromosomal deletion Δ431[33] that removes genes rightward from ninB in prophage through moaA in host, including prophage genes orf146 (orf) – Jb2 (i.e., all the late genes required for cell lysis and phage morphogenesis). A map of the cryptic lambda prophage in strain Y836 is drawn in Fig. 4A.bLysogenic strains show the prophage within the cell by “( )” bordering the prophage.cThe NinR region deleted by Δnin5 removes λ bases 40,503–43,307, i.e., ren-ninA – ninI (including orf -ninC and rap-ninH ); the NinL region substituted by bio275 replaces genes int-xis-hin-exo-bet-gam-kil, representing λ bases 27,731–∼33,303 [18].
创建时间:
2015-12-02



