Prabuddha Sengupta (2010) CIL:9157, Homo sapiens, permanent cell line cell, epithelial cell. CIL. Dataset

Sialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA).
Images were collected as a 4D time series (z-stack over time). z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points.

BFA was added according to the following protocol:
Timepoint1: Before addition of BFA 0seconds (8 Z-slices at 1 um apart)
Timepoint2: After addition of BFA 130seconds (8 Z-slices at 1 um apart)
Timepoint3: 238 seconds (11 Z-slices at 1 um apart)
Timepoint3 thru Timepoint48: Images captured every 5 seconds (11 Z-slices at 1 um apart at each time point)

Images were collected on a Carl Zeiss spinning disk confocal with a 63X 1.4 NA objective with 250 ms exposures at 5 s intervals.

Sialyl转移酶-EGFP转染的人HeLa细胞在经Brefeldin A（BFA）处理后，展现了高尔基体向内质网的重新分布现象。图像以四维时间序列（随时间变化的z-stack）形式收集。本数据集包括48个独立时间点的z-stack以及SiEGFP_BFA.avi电影，该电影为单个时间点z-stack的最大投影。BFA的添加遵循以下方案：时间点1：在添加BFA前的0秒（8张间隔为1微米的Z切片）；时间点2：添加BFA后的130秒（8张间隔为1微米的Z切片）；时间点3：238秒（11张间隔为1微米的Z切片）；时间点3至时间点48：每个时间点每隔5秒捕获图像（每个时间点11张间隔为1微米的Z切片）。图像使用卡尔·蔡司旋转盘共聚焦显微镜，配备63倍1.4NA物镜，以250毫秒曝光，每隔5秒进行一次采集。