Single-cell RNA sequencing unveils fibroblast heterogeneity in proliferative vitreoretinopathy membranes

Proliferative vitreoretinopathy (PVR) is a vision-threatening fibrotic disorder characterized by pathological membrane formation, yet its cellular and molecular mechanisms remain incompletely understood. Here, we employed single-cell RNA sequencing (scRNA-seq) to profile 18,213 cells from 5 human PVR membranes (PVRMs), uncovering cellular heterogeneity and novel pathogenic pathways. Overall design: BD Rhapsody system was used to capture the transcriptomic information of the single cells. Single-cell capture was achieved by random distribution of a single-cell suspension across >200,000 microwells through a limited dilution approach.