Optogenetics_Dfd_population_imaging

This dataset contains Dfd population imaging data and behavioral including responses to optogenetic stimulation. Part of Braun et al. 2023. There are two different types of tar.gz files. The one ending with "processed.tar.gz" contains data related to one fly: 1. "background_image.tif" is a standard deviation projection of raw fluorescence data used as background in figure 2c. 2. "roi_center_annotation.pdf" is a plot indicating the results of semi-automated ROI detection. 3. "ROI_centers.txt" indicates the location of said ROI centers. 4. "ROI_mask.tif" is the mask used for ROI extraction for this fly. ------------------- The other tar.gz files each correspond to one 10min long recording of neuronal activity and behavior of one fly. If multiple recordings have been made in one fly, their sequence is indicated with a 3 digit number in the name. Each of those folders contains 3 subfolders: "2p" holds the synchronisation data and metadata and the two photon recording metadata. "behData" contains the behavioral camera metadata. "processed" contains a pickled pandas dataframe containing all processed behavioral variables (beh_df.pkl) and all neuronal time series (twop_df.pkl) required to reproduce the figures. Raw behavioral videos and raw fluorescence data are available upon request from the authors and are omitted here because of their size.