lam lethality

Stages of lethality for different lam alleles. 200 larvae of each genotype were selected and scored for survival as described in Materials and Methods. Numbers refer to the percentage of animals that died during larval and pupation stages, and the percentage of adult escapers that emerged. Extensive counts of larval emergence did not reveal marked embryonic lethality for any of the lam alleles (data not shown). Wild type flies were w1118 (Oregon R). Our observing only 83% wild type adult survival compared to expectations of 99% could be explained by culture conditions or trauma during handling, but as all genotypes were handled similarly, this did not bias results. Note the slight difference between lamD395 homozygotes and lamD395/Df (2L) cl-h1 transheterozygotes, which is discussed in the text. * No lamp pupae were observed in this experiment, but 5 lamp larvae survived to pupate from a much larger pool in our pupariation height experiment, shown in Figure 3B.