Pollen DNA metabarcoding reveals high diversity and spatial turnover in alpine plant-pollinator networks

ITS2 and trnL amplicon reference database for alpine plants surveyed at Charlotte's Pass, Kosciuszko. \nLineage: Reference database: DNA extracted from leaf tissue taken from herbarium specimens from Australian National Herbarium (ANH) or collected from field near Charlotte's Pass, Kosciuszko National Park, NSW. Data was sequenced by Sanger and NGS (MiSeq) at Ramaciotti Centre For Genomics (UNSW), with funding from Bio Platforms Australia. \nPrimers used are: \nITS2-S2F:ATGCGATACTTGGTGTGAAT, Chen, et al. (2010) \nITS2-4 rev:TCCTCCGCTTATTGATATGC, White, et al. (1990)\ntrnL (UAA) fwd: \ntrnL (UAA) rev: \n\nPollen metabarcoding data: \ntrnL (UAA) p6 loop-g:GGGCAATCCTGAGCCAA, Baamrane, et al. (2012) \ntrnL (UAA) p6 loop-h:CCATTGAGTCTCTGCACCTATC, White, et al. (1990)