64 primer pairs developed and tested in silico for Salminus brasiliensis
收藏doi.org2025-01-21 收录
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http://doi.org/10.17632/wh3nsdz4hn.1
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The optimization 137 temperature was evaluated, and it was possible to select even more robust primers, yielding 64 primers pairs this value was obtained manually by the OligoAnalyser website that evaluates the presence of harpins and dimers of each primer.
对137温度下的优化进行了评估，并成功筛选出更为稳定的引物，最终得到64对引物。此数值系通过OligoAnalyser网站（该网站评估各引物的环状结构和二聚体存在情况）手动获得。
提供机构：
Mendeley Data
