Digital holograms of isolated HL60 cell nuclei

<b>Digital holograms of isolated HL60 cell nuclei<br></b><br>These data were originally used by Schürmann et al. [1] for the<br>quantification of the refractive index of isolated HL60 cell nuclei.<br><br><br>Raw data<br>The data are related to [1] according to the following scheme<br>(last part before suffix indicates cell cycle stage):<br><br>Figure 5 - 1st measurement:<br>2014-05-29_DHM_HL60-nuclei-G1.zip<br>2014-05-29_DHM_HL60-nuclei-G2.zip<br>2014-05-29_DHM_HL60-nuclei-S.zip<br><br>Figure 5 - 2nd measurement:<br>2014-07-03_DHM_HL60-nuclei-G1.zip<br>2014-07-03_DHM_HL60-nuclei-G2.zip<br>2014-07-03_DHM_HL60-nuclei-S.zip<br><br><br>Data analysis<br>Data analysis in [1] has been performed largely manual. In [2],<br>the analysis has been revisited with DryMass [3] which enables an<br>automated data analysis. The .cfg files are DryMass configuration<br>files [4], i.e. analysis recipes that allow a complete data analysis<br>from scratch. The file "HL60nuc.cfg" contains the basic data analysis<br>recipe. The file "ignore_ROIs.txt" contains the ignored regions of<br>interest for each measurement. The other .cfg files contain the same<br>information with default values filled in by DryMass. The analysis<br>has been tested with DryMass version 0.10.0.<br><br>To run an analysis, issue e.g. the following command:<br><br> dm_analyze_sphere -p 2014-05-29_DHM_HL60-nuclei-G1.cfg 2014-05-29_DHM_HL60-nuclei-G1.zip<br><br><br>References<br>[1] M. Schürmann et al., "Cell nuclei have lower refractive index and mass density than cytoplasm," Journal of Biophotonics 9(10): 1068–1076, 2016. doi:10.1002/jbio.201500273.<br>[2] P. Müller et al., "DryMass: handling and analyzing quantitative phase microscopy images of cell-sized objects," manuscript in preparation.<br>[3] https://drymass.readthedocs.io/<br>[4] https://drymass.readthedocs.io/en/stable/sec_gs_configuration_file.html<br><br>