MOESM1 of Plasmodium falciparum parasite population structure and gene flow associated to anti-malarial drugs resistance in Cambodia

Additional file 1. Geographic distribution of samples and allele frequency. Blood samples from P. falciparum positive patients were collected from 16 health centres or hospitals covering the areas where parasite transmission is active. The number of valid samples (282) out of the total selected samples (533) is provided per health centre. The frequency of each allele (REF/ALT) is given for the 11 loci, which were positive for LUMINEX detection. The alleles with Chi squared test statistics components (one component for each health centre) greater than 1 are highlighted in grey. The presence of Kelch-propeller domain altered allele was assessed by PCR and sequencing. The frequencies of wild type individuals and of the two major alleles C580Y and R539T are provided per location. The other alleles present at low frequency, N458Y, Y493H, I543T, P553L and V568G are pooled together. The drug sensitivity was measured routinely for patients presenting high parasitaemia (>2Â %). The number of samples tested for IC50 measurements for chloroquine (CQ), piperaquine (PIP), quinine (QN), artesunate (ART), mefloquine (MF) and dihydroartemisinin (DHA) is provided.