Transcriptome of bovine trophoblast stem cells (TSC) - in self-renewal and differentiation

In this study, we examine the transcriptome of bovine TSCs under self-renewal that is sustained by inhibiting RhoA-Rock pathway. Using systems biology of spontaneous differentiation, we identify that TGFB1 induces differentiation of bovine TSCs. We analyse the transcriptome of TSCs under TGFB1 induced differentiation. We also analyze pathways of differentiation that are dependent and independent of RhoA-Rock signaling through analysis of the transcriptome of TSCs exposed to both TGFB1 and ROCK inhibition. Undifferentiated bovine blastocyst-derived trophectoderm cells from IVF embryos were cultured, and changes to the transcriptome were examined after exposure to RhoA-Rock inhibition and TGFB1 stimulation.