Targeting VRK2 Enhances the Efficacy of Anti-PD-1 Therapy in Hepatocellular Carcinoma by Reducing Direct Phosphorylation and Stability of MYC,CUT-TAG sequencing for MYC in hepatocellular carcinoma cells

VRK2–MYC axis, a transcription-dependent regulatory axis, could be a prognostic indicator and potential therapeutic target in liver cancer. Overall design: Identify downstream target genes regulated by VRK2 or MYC in MHCC97H cells using whole transcriptome sequencing (RNA-Seq). The expression of MYC or VRK2 was interfered with using siRNA in MHCC97H