Neutralizing antibody titers in mice immunized with D1ED III formulated with PELC plus CpG before and after viral challenge1.

1Groups of BALB/c mice (n = 5) were immunized three times subcutaneously with 3, 10, or 30 µg of D1ED III formulated with PELC plus CpG at two-week intervals. Naïve mice without immunization served as controls. All animals were inoculated intraperitoneally with 3×106 FFU of live dengue-1 virus at week 26 post-priming. Sera were collected at different time points as indicated. The sera's ability to neutralize dengue-1 virus was determined by FRNT. The neutralizing antibody titer was calculated as the reciprocal of the highest dilution that resulted in a 50% or 80% reduction of FFU compared to control samples containing the virus alone.2Statistical analysis was performed by the ANOVA Bonferroni post test at the same time point.*p#p§p