WGS on four cell lines with Element AVITI and Illumina NovaSeqX+

To compare Element to Illumina sequencing for WGS, a pool of PCR-free gDNA libraries was sequenced on both Element AVITI and Illumina NovaSeqX+ instruments. Illumina-style PCR-free WGS libraries were prepared using DNA from 4 cell lines, 3 of multiple myeloma origin (MM1S, OPM2, KMS12BM) and one acute lymphocytic leukemia cell line (REH). The same library pool was sequenced using both Illumina NovaSeq X Plus and Element AVITI sequencing instruments. For Illumina sequencing (NovaSeq X+), the pool was loaded on two 10B flowcell lanes on a NovaSeq X Plus instrument. Two rounds of Element AVITI sequencing were performed. In the first round (AVITI CB), libraries were converted to circular Element-style libraries and loaded onto two Element Cloudbreak flow-cells for sequencing. In the second round (AVITI CB FS), libraries were directly loaded on a single Element Cloudbreak Freestyle flowcell for on-board circularization and sequencing