Transcriptional changes between mono- and dual-species biofilms of Pseudomonas aeruginosa and Candida albicans, ± meropenem treatment

The goals of this dual-seq experiment were to 1) identify transcriptional changes between mono-species and dual-species biofilms of Candida albicans and Pseudomonas aeruginosa and 2) identify transcriptional changes within mono- or dual-species P. aeruginosa biofilm cells in response to meropenem treatment. Mono- and dual-species biofilms of C. albicans and P. aeruginosa were grown in vitro, with or without meropenem treatment. Four biological replicates were included, with six samples per replicate. For transcriptional analyses, the reference genomes used were: P. aeruginosa PAO1 (https://www.ncbi.nlm.nih.gov/nuccore/NC_002516.2) and C. albicans SC5314 Assembly 21 (http://www.candidagenome.org/download/sequence/C_albicans_SC5314/Assembly21/current/).