Transcriptomics analysis of gene expression in wildtype and NSUN2 knockdown T24 cell. Transcriptomics analysis of gene expression in wildtype and NSUN2 knockdown T24 cell

Total RNA was isolated using TRIzol® Reagent (Ambion). The RNA concentration and quality were determined with NanoDrop, Qubit and agarose gel analysis. The Dynabeads® mRNA Purification Kit (Ambion) was used to enrich the mRNAs, which were used as templates for RNA-Seq library construction. RNA-Seq libraries was directly constructed by using KAPA mRNA Stranded mRNA-Seq Kit (KAPA) according to the manufacturer’s instructions. The libraries were sequenced on the Illumina HiSeq X-Ten platform at Novogene (Tianjin, CA) with paired-end 150 bp read length. The different genes expression between the control and NSUN2 knockdown cell were detected. Overall design: Examination of gene expressive levels in wildtype and NSUN2 knockdown T24 cell